
I recently got a
DNA11 Mini-Portrait. The "DNA Portrait Explained" booklet mentions the DNA sequences analyzed. I thought these referred to restriction enzymes, but it looks like they are actually primers for
RAPD (random amplification of polymorphic DNA). Below are the lanes, the DNA sequences, and the RAPD primer codes:
Lane 1 & 10: Molecular rulers
Lane 2: GTGAGGCGTC, OPC-02
Lane 3: AGCGCCATTG, OPD-11
Lane 4: GTGTGCCCCA, OPD-08
Lane 5: TGCGGCTGAG, OPE-14
Lane 6: AGTCAGCCAC, OPA-03
Lane 7: GTGATCGCAG, OPA-10
Lane 8: AGGTGACCGT, OPA-18
Lane 9: GTCGCCGTCA, OPD-03
There are only 6 lanes in the DNA11 mini-portrait, so it is unclear which subset of those primers are used in the mini-portrait. I'll have to email them!
Update: DNA11 says "The markers utilized for mini gels are OPC-02, OPD-03, OPD-08, and OPD-11. However, the order in which they appear in the lanes is randomized to ensure that even closely related individuals get a very unique portrait. We do this for mini and full size portraits."